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taqtth plasmid map  Taqtth Plasmid Map, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/taqtth plasmid map/product/Benchling Inc Average 86 stars, based on 1 article reviews
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2026-05
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Journal: Nucleic Acids Research
Article Title: DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway
doi: 10.1093/nar/gkaf723
Figure Lengend Snippet: The design of the TaqTth-hpRNA and validation in vitro . ( A ) The domain organization and predicted three-dimensional structure of TaqTth. Domains from TaqPol (1-328 aa and 595-832 aa) are shown in yellow. The domain from TthPol (331-596 aa) is shown in gray. ( B ) TaqTth cleavage of targeted ssDNA guided by different types of probes. The probes were hairpin DNA probe (hpDNA, probe 1), RNA probe with 3′ flap structure (probe 2), hairpin guide RNA probe with 3′ flap structure (hpRNA, probe 3), RNA probe with 5′ flap structure (probe 4), and hairpin guide RNA probe with 5′ flap structure (probe 5). ( C ) The extension of stem-loop in hpDNA and hpRNA by TaqTth in vitro . ( D ) The scheme and result of the sequence preference of TaqTth-hpRNA in vitro . ( E ) The scheme and result of the cleavage site of TaqTth-hpRNA in vitro . ( F ) TaqTth cleavage of targeted ssDNA guided by different types of stem-loop hpRNAs (hpRNA-V1, hpRNA-V2, and hpRNA-V3). ( G ) Effect of guide sequences with various lengths ranging from 25 to 10 nt at an interval of 5 nt.
Article Snippet: The
Techniques: Biomarker Discovery, In Vitro, Sequencing
Journal: Nucleic Acids Research
Article Title: DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway
doi: 10.1093/nar/gkaf723
Figure Lengend Snippet: TaqTth-mediated DNA cleavage in E. coli . ( A ) The plasmid maps of the p-TaqTth, p-hpRNA-S, and p-hpRNA-A. TaqTth was induced by tetracycline. ( B ) The schematic diagram of the tests for TaqTth-mediated genome editing in E. coli . ( C ) The rate of clone survival when targeting the sense or antisense strand of arpB in MG1655; mean ± s.d., n = 3. ( D ) The rate of clone survival in MG1655 after expression of the three-plasmid system (p-TaqTth, p-hpRNA-S, and p-hpRNA-A) targeting DNA double strands of arpB ; mean ± s.d., n = 3, ** P < .01. ( E ) The rate of clone survival in MG1655 after expression of the dual plasmid system (p-TaqTth and p-biohpRNA) targeting DNA double strands of arpB ; mean ± s.d., n = 3, ** P < .01. ( F ) The rate of clone survival in MG1655 after expression of the dual plasmid system (p-TaqTth and p-biohpRNA) targeting DNA double strands of sulA promoter; mean ± s.d., n = 3, **** P < .0001. ( G ) The scheme and result of the sequence preference of TaqTth-hpRNA in E. coli .
Article Snippet: The
Techniques: Plasmid Preparation, Expressing, Sequencing
Journal: Nucleic Acids Research
Article Title: DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway
doi: 10.1093/nar/gkaf723
Figure Lengend Snippet: TaqTth-hpRNA facilitates large deletions at endogenous targeted loci in HEK293T cells. ( A , B ) The location of hpRNAs for the endogenous CCR5 and EMX1 loci, the typical Sanger sequencing results of the TaqTth-hpRNA-edited product with large deletions, and agarose gel electrophoresis of colony PCR. ( C ) The deletion efficiency at the endogenous CCR5 and EMX1 loci via qPCR in HEK293T cells; mean ± s.d, n = 4. ( D ) The hypothesis of large deletions assisted with MMEJ by TaqTth-hpRNA.
Article Snippet: The
Techniques: Sequencing, Agarose Gel Electrophoresis
Journal: Nucleic Acids Research
Article Title: DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway
doi: 10.1093/nar/gkaf723
Figure Lengend Snippet: Analysis of allele-specific genome editing of CRISPR/Cas9 and TaqTth-hpRNA. ( A ) The schematic view of assays of APP wt and 5×FAD. ( B , C ) The schematic diagram of CRISPR/Cas9 assay with gRNAs targeting the APP swe and APP lon mutations. Effect of CRISPR/Cas9 on mRNA levels of APP in the HEK293T-5×FAD and HEK293T-APP-WT cell lines; mean ± s.e.m., n = 3, * P < .05, ns means no significant difference. ( D , E ) The schematic diagram of TaqTth assay with hpRNAs targeting the APP lon single-nucleotide mutation. Relative APP knockdown efficiencies on mRNA level induced by TaqTth-hpRNA in the HEK293T-5×FAD and HEK293T-APP-WT cell lines; mean ± s.e.m., n = 3, * P < .05. ( F ) The silencing efficiencies of APP lon and APP wt on protein level induced by TaqTth-hpRNA. ( G ) The typical Sanger sequencing results of the TaqTth-hpRNA-edited product with large deletions in the HEK293T-5×FAD cell line.
Article Snippet: The
Techniques: CRISPR, Mutagenesis, Knockdown, Sequencing